Alternate Spray for Organo - Phosphorous Compound for Quick and Better Screening

 

Kamalakshi Krishnamurthy1, Thant Zin2, K. Priyamvatha3 Mahadeva Rao.U.S.2,

Suganya M4, K. Mariappan5

1Additional Director and Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai, India.

2School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA),

Kuala Terengganu, Malaysia.

3Former Student of Women’s Christian College, Chennai, India.

4People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India. 

5Assistant Director (R&D Division), Forensic Sciences Department, Mylapore, Chennai, India.

*Corresponding Author E-mail: raousm@gmail.com, raousm@unisza.edu.my

 

ABSTRACT:

Organo-Phosphorous compounds are isolated and located on Thin Layer chromatography by brominating the spotted, eluted plate with subsequent spray of Congo Red. In the above method, the presence of unavoidable co-extracts from the visceral matter interferes leading to misconception and false positive results. This paper overcomes the above drawback by finding a new spray in Rhodamine 6G.

 

KEYWORDS: Rhodamine 6G, Organo-Phosphorous, Congo Red, fluorescent.

 

 

INTRODUCTION:

Rhodamine 6 G is a highly fluorescent dye pertaining to Rhodamine family with a formula C28 H31 N2O3Cl.  Has an absorption around 500-550 nm with the maximum at 530 nm. It is extensively used in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation Spectroscopy and ELISA.

 

Rhodamine 6 G chloride is a bronze/red powder soluble in ethanol, methanol, butanol, propanol and dipropylene glycol etc. It is also used as a laser dye, has a high photostability, high fluorescence quantum yield and has a low cost.

 

Methods:

Chemicals:

Silica Gel GF 254, Rhodamine 6 G

System for Elution:

Hexane: Acetone: 4:1

 

 

Methyl Parathion control is spotted in the plate and is allowed to run to sufficient height in the saturated solvent system. Plate removed and dried. Sprayed with 0.1% Rhodamine 6G in Rectified spirit or Acetone. Pink spots on a saffron background revealed. Plate viewed under Ultra-Violet light. Spots fluoresces as Violet.

 

Now the TLC plate is brominated. Oils, fats and other co-extracts give golden yellow fluorescence whereas Methyl Parathion gives a deep violet colour thus enabling us to differentiate from other co-extracts. Thus, this spray plays a very important role in identifying Methyl Parathion and other organo-phosphorous compounds from its co-extracts.

 

REFERENCES:

1.      Casarette and Doull's Toxicology - Basic Science of Poisons - Curtis D. Klassen - 5th edition.

2.      Essentials of Forensic Medicine and Toxicology – Narayan Reddy and O.P. Murthy.

3.      Parikh's Textbook of Medical Jurisprudence and Toxicology- C.K. Parikh.

4.      Textbook of Medical Jurisprudence and Toxicology-Jaising Modi.

5.      Isolation and Identification of Drugs in Pharmaceuticals, body fluids and Post-Mortem Material-E. C. G. Clarke.

6.      Text book of Organic Chemistry - P.L. Soni and H.M. Chawla.

 

 

Received on 10.05.2022                    Modified on 08.06.2022

Accepted on 28.06.2022                   ©AJRC All right reserved

Asian J. Research Chem. 2022; 15(3):220.

DOI: 10.52711/0974-4150.2022.00038